Cureline research team has adopted effective protocols for preparation of primary human cell lines from solid tumors, cancer blood, cancer bone marrow and normal tissues. The prepared primary cell cultures maintain their differential state and can be used as an in-vitro model system to study relevant disease changes and susceptibility to new drug candidates.
Our scientists can prepare primary cell lines from most tissues using our standard protocols. If you would like to implement a custom project, please contact Cureline Biospecimene Operations team in order to discuss your project specifications.
APPLICATION: Analysis of drug sensitivity and target expression levels
Rheumatoid Arthritis: Primary cell culture from cells contained in synovial fluid of treatment-naïve RA patients vs patietns treated with biologic drugs (Humira, Enbrel, etc.)
CLL: Preparation of total and CD19+ viable PBMCs
MM: Preparation of total and CD138+ viable BMMCs PBMCs
SLE: Preparation of PBMCs and treatment with client's drug candidate
APPLICATION: Discovery of cancer - relevant antigens
Spleen Primary Cell Line: Preparation of a primary cell line from normal human spleen tissue