Clinically-Defined HBS from Patients with AID
Rheumatoid Arthritis (RA),a chronic autoimmune disorder causing joint inflammation and damage, is one of the most studied indications in recent years. Cureline standard procurement protocols and processes for collection of synovial tissue and synovial fluid from hand/elbow and knee/hip joints from patients with various stages of RA fulfill the quality requirements for most research applications used in drug discovery preclinical research drug safety evaluation, biomarker development and clinical assay validation.
Cureline's team designs and implements custom tissue procurement and research protocols including:
- Collection of snap-frozen synovial tissue and synovial fluid
- Collection of matching rheumatoid arthritis serum, plasma, whole blood and PBMCs
- Target expression and activation profiling in synovial tissue using IHC
- Development of primary cell culture of synovial fluid cells
Standard Specimen Format and Sets
- Synovial tissue snap-frozen biopsy
- Synovial tissue matching FFPE biopsy
- Synovial fluid:
- frozen synovial liquid fraction
- viable synovial fluid cells
- Matching rheumatoid arthritis serum
- Matching rheumatoid arthritis plasma
- Whole blood in PAXgene RNA tube
APPLICATION: Stress-response kinase activation study
Immediately snap-frozen samples: Collection of synovial fluid synovial tissue (biopsy or surgical material) and urine samples from RA and normal control patients. All samples were snap-frozen within 2 minutes after procurement.
APPLICATION: Comparative expression study using protein lysates and RNA
Specimens from patients with differential treatments: Preparation of snap-frozen and paraffin-embedded synovial tissue frozen synovial fluid and RNA-ready blood from RA patients with differential treatments (anti-TNFα medications vs others).
APPLICATION: Study testing drug sensitivity and expression levels
Primary cell culture from synovial fluid: Preparation of viable cells from synovial fluid of treatment-naïve RA patients.