Cureline has established collaborations for the collection of biospecimens and the preparation of human RNA and DNA panels from diseased and normal tissues. Our new laboratory, established in San Diego, (CA, USA) in 2015, provides RNA, DNA and protein isolation services using Cureline biorepository samples or tissues/biofluids submitted by a client.
 
Our standard research protocols for human RNA/DNA isolation fulfill the quality requirements for most research methods, including NGS, SNP variations and differential expression analysis using different microarray platforms.

Project Examples

Lung cancer - adenocarcinoma and SCC:
Preparation of high-quality total human RNA from snap-frozen tumor and ANT specimens
 
Breast Carcinoma and NCSLC (adenocarcinoma and SCC):
Preparation of high-quality total human RNA from matching tumor tissue (at least 70% tumor content) and ANT for Affimetrix gene expression analysis (data were presented at the annual EORTC-AACR-NCI conference in San Francisco, October 2007)
 
Prostate cancer:
Preparation of high-quality total human RNA from snap-frozen tumor and ANT specimens
 
Normal human tissues:
Preparation of high-quality DNA-free total human RNA from snap-frozen tissue samples (panel of 20 organs)