Cell Fractions from Human Biospecimens
Cureline’s research team has developed protocols for preparation of primary human viable cells and and snap-frozen cell pellets for in-vitro functional studies and protein and RNA expression analysis.Our scientists can prepare viable primary cells and frozen cell fractions from tumor and normal tissues, blood and bone marrow. If you would like to implement your custom protocol, please contact Cureline’s Scientific Director, Olga Potapova, Ph.D., to discuss your project specifications.
Recent Collections
APPLICATION: Functional in vitro studies (signal transduction, apoptosis, cell viability) using a new drug candidate.
Leukemia (CLL, CML, ALL, AML):
Isolation of PBMCs from fresh blood using a Ficoll-based procedure, and preparation of viable human cells in DMSO-containing medium. Collection of matching cancer serum samples and extensive clinical information for each patient.
Chronic Lymphocytic Leukemia (CLL):
Isolation of CD19+ human cells cells from fresh blood using magnetic beads and preparation of viable primary cells in DMSO-containing medium. Collection of matching cancer serum samples and extensive clinical information for each patient
Multiple Myeloma (MM):
Preparation of viable primary cultures of CD138+ cells isolated from fresh bone marrow aspirate using magnetic beads.
Myelodysplastic Syndrome (MDS):
Preparation of viable human BMMCs, snap-frozen cell pellets and RNA. Collection of matching serum samples and extensive clinical information for each patient
